Selected Publications (alphabetical)

Alexander, R.T.

Alexander R.T., Cordat E., Chambrey R., Dimke H., Eladari D. (2016) – “Acidosis and urinary calcium excretion, insights from genetic disorders”. In press for Journal of the American Society of Nephrology.

Krishnan D, Liu L, Wiebe SA, Casey JR, Cordat E, Alexander RT. (2015) Carbonic anhydrase II binds to and increases the activity of the epithelial sodium-proton exchanger, NHE3. Am J Physiol Renal Physiol. 309(4):F383-92.

Vilas G, Krishnan D, Loganathan SK, Malhotra D, Liu L, Beggs MR, Gena P, Calamita G, Jung M, Zimmermann R, Tamma G, Casey JR, Alexander RT. (2015) Increased water flux induced by an aquaporin-1/carbonic anhydrase II interaction.. Mol Biol Cell. Mar 15;26(6):1106-18.

Alexander RT, Beggs MR, Zamani R, Marcussen N, Frische S, Dimke H. (2015) Ultrastructural and Immunohistochemical Localization of the Plasma Membrane Ca2+-ATPase 4 (PMCA4) in Ca2+-Transporting Epithelia. Am J Physiol Renal Physiol. Jul 15:ajprenal.00651.2014. doi: 10.1152/ajprenal.00651.2014.

Alexander RT, Hemmelgarn BR, Wiebe N, Bello A, Samuel S, Klarenbach SW, Curhan GC, Tonelli M. (2014) for the Alberta Kidney Disease Network.  Kidney stones and cardiovascular events:  a cohort study.  Clin J Am Soc NephrolMar;9(3):506-12.

Fuster DG and Alexander RT. (2014) Traditional and Emerging Roles of the SLC9 Na+/Hexchangers. Pflugers Arch Jan;466(1):61-76.

Dimke H, Desai P, Borovac J, Lau A, Pan W, Alexander RT. (2013) Activation of the Ca2+-Sensing Receptor Increases Renal Claudin-14 Expression and Urinary Ca2+ Excretion.  Am J Physiol Renal Physiol Mar 15;304(6):F761-9.

Alexander RT, Dimke H, Cordat E. (2013) Proximal Tubular NHEs:  Sodium, Protons and Calcium?  Am J Physiol Renal Physiol Aug;305(3):F229-36.

Rievag J, Pan W, Cordat E, Alexander RT. (2013) The Na+/H+ Exchanger Isoform 3 is required for active paracellular and transcellular Ca2+ transport across murine caecum.  Am J Physiol Gastrointest Liver Physiol Aug;305(4):G303-13.

Pan W, Borovac J, Spicer Z, Hoenderop JG, Bindels RJ, Shull GE, Doschak MR, Cordat E, Alexander RT. (2012) The Epithelial Sodium Proton Exchanger, NHE3, is necessary for renal and intestinal calcium (re)absorption. Am J Physiol Renal Physiol, 2(8):F943-56.  (I am senior and corresponding Author).

Samuel SM, Foster BJ, Tonelli MA, Nettel-Aguirre A, Soo A, Alexander RT, Hemmelgarn BR; (2012) Association between distance from renal care center and death in pediatric end-stage renal disease patients. Pediatr Transplant  Nov;16(7):735-41.

Alexander RT, Foster BJ, Tonelli MA, Soo A, Nettel-Aguirre A, Hemmelgarn BR and Samuel SM; (2012) the Pediatric Renal Outcomes Canada Group. Outcomes of Children Less than 2 years of Age initiating dialysis. Pediatr Nephrol. Oct;27(10):1975-83.

Almomani EY, King JC, Netsawang J, Yenchitsomanus PT, Malasit P, Limjindaporn T, Alexander RT, Cordat E. (2012) Adaptor protein 1 complexes regulate intracellular trafficking of the kidney anion exchanger 1 in epithelial cells. Am J Physiol Cell Physiol. Sep;303(5):C554-66.

Samuel SM, Foster BJ, Hemmelgarn BR, Nettel-Aguirre A, Crowshoe L, Alexander RT, Soo A, Tonelli MA. (2012) Incidence and causes of end-stage renal disease among Aboriginal children and young adults. CMAJ.  Oct 2;184(14):E758-64.

Borovac J, Barker RS, Rasmussen A, Pan W, Wevrick R and Alexander RT. (2012) Claudin-4 forms a paracellular barrier, revealing the interdependence of claudin expression in the loose epithelial cell culture model opossum kidney cells. Am J Physiol Renal Physiol,  Dec;303(12):C1278-91. (I am Corresponding Author).

Alexander RT, Jaumouillé V, Yeung T, Furuya W, Peltekova I, Boucher A, Zasloff M, Orlowski J, Grinstein S.EMBO J. (2011) Membrane surface charge dictates the structure and function of the epithelial Na+/H+ exchanger. 30(4):679-91.


Casey, J.R.

Badior, K., Alka, K., and Casey, J.R. (2016) SLC4A11 Three-Dimensional Homology Model Rationalizes Corneal Dystrophy-causing Mutations, Human Mutation, In press.

Loganathan, S.K., Schneider, H.P., Morgan, P.E., Deitmer, J.W., and Casey, J.R. (2016) Functional Assessment of SLC4A11, an Integral Membrane Protein Mutated in Corneal Dystrophies, Am. J. Physiol., 311, C735-C748.

Reithmeier, R.A.F., Casey, J. R., Kalli, A., Sansom, M.S.P. Alguel, Y., and Iwata, S. (2016) Band 3, the Human Red Cell Chloride/Bicarbonate Anion Exchanger (AE1, SLC4A1), in a Structural Context Biochim. Biophys. Acta, 1858, 1507-32

Chiu, A.M., Mandziuk,  J.J., Alka, K., Loganathan, S.K., and Casey, J.R. (2015) High Throughput Assay Identifies Glafenine as a Chemical Corrector for the Folding Defect in Corneal Dystrophy-Causing Mutants of SLC4A11, Invest. Opthal. and Vis. Sci., 56, 7739-53.

Loganathan, S.K., Lukowski, C.M. and Casey, J.R. (2016) The cytoplasmic domain is essential for transport function of the integral membrane transport protein SLC4A11, Am. J. Physiol.310, C161-174.

Krishnan, D., Liu, L., Wiebe S.A., Casey, J.R., Cordat, E. and Alexander, R.T. (2015) Carbonic anhydrase II binds to and increases the activity of the epithelial sodium proton exchanger, NHE3, Am. J. Physiol., 309, F383-92.

Zoccola, D., Ganot, P., Bertucci, A., Caminiti-Segonds, N., Techer, N., Voolstra, C., Aranda, M., Tambutté, E., Allemand, D., Casey, J.R. and Sylvie Tambutté (2015) Bicarbonate Family Transporters In A Scleractinian Coral, Scientific Reports. 5, 2204-08.

Vilas, G.L., Krishnan, D., Loganathan, S.K., Malhotra, D., Liu, L., Beggs, M., Gena, P., Calamita, G., Jung, M., Zimmermann, R., Tamma, G., Casey, J.R. and Alexander, R.T. (2015), Increased Water Flux Induced by an Aquaporin-1/Carbonic Anhydrase II interaction, Mol. Biol. of Cell., 26, 1106-18.

Okawa, Y., Li, J., Basu, A., Casey, J.R. and Reinhart A.F. Reithmeier (2014) Differential Roles of Tryptophan Residues in the Functional Expression of Human Anion Exchanger 1 (AE1, Band 3, SLC4A1) Molecular Membrane Biology, 26, 1-17.

Sowah, D., Brown, B.F., Quon, A., Alvarez, B.V., and Casey, J.R. (2014) Resistance to Cardiomyocyte Hypertrophy in ae3-/- Mice, Deficient in the AE3 Cl-/HCO3- Exchanger, BMC Cardiovascular Disorders14:89.

Loganathan, S.K., and Casey, J.R. (2014) Corneal Dystrophy-causing SLC4A11 Mutants: Suitability for Folding-Correction Therapy, Human Mutation35, 1082-91. (Article selected for a video highlight; See

Soumittra, N., Loganathan, S.K., Madhavan, D., Ramprasad, V.L., Arokiasamy, T., Sumathi, S., Karthiyayini, T., Rachapalli, S.R., Kumaramanicakvel, G., Casey, J.R., and Rajagopal, R. (2014), Biosynthetic and functional defects in newly-identified SLC4A11 mutants and absence of COL8A2 mutations in Fuchs endothelial corneal dystrophy, J. Human Genetics, 59, 444-53.

Shnitsar, V., Li, J., Li, X., Calmettes, C., Basu, A., Casey, J.R., Moraes, T.F. and Reithmeier, R.A.F. (2013) Structural and Functional Analysis of a Putative Substrate Access Tunnel in the Cytosolic Domain of Human Anion Exchanger 1 (AE1, Band 3, SLC4A1), J. Biol. Chem288, 33848-60.

Vilas, G.L., Loganathan, S., Liu J., Riau, A.K., Young, J.D., Mehta, J.S., Vithana, E.N. and Casey, J.R. (2013) Transmembrane water flux through SLC4A11: a route defective in corneal diseases, Human Mol. Genet., 22, 4579-90.

Bonar, P.T., Schneider, H.P., Becker, H.M., Deitmer, J.W., and Casey, J.R. (2013) Three-Dimensional Model for the Human Cl-/HCO3- Exchanger, AE1, by Homology to the E. coli ClC Protein J. Mol. Biol. 425, 2591-2608.

Alvarez, B.V., Quon, A., Mullen, J. and Casey, J.R. (2013) Quantification of carbonic anhydrase gene expression in ventricle of hypertrophic and failing human heart, BMC- Cardiovascular Disorders, 13:2.

Brown, B., Quon, A., Dyck, J. and Casey, J.R. (2012) Carbonic Anhydrase II Promotes Cardiomyocyte Hypertrophy, Can. J. Physiol. Pharmacol. 90, 1599-610.

Vilas, G.L., Loganathan, S., Quon, A.,, Sundaresan, P., Vithana, E.N and Casey, J.R. (2012) Oligomerization of SLC4A11 protein and the severity of FECD and CHED2 corneal dystrophies caused by SLC4A11 mutations, Human Mutation33, 419-428.


Chen, X-Z.    

Zheng, W., Shen, F., Hu, R., Roy, B., Yang, J., Wang, Q., Zhang, F., King, J.C., Sergi, C., Liu, S.-M., Cordat, E., Tang, J., Cao, Y., Ali, D.W., and Chen, X.-Z.* Far upstream element-binding protein 1 binds the untranslated region of PKD2 and suppresses its translation. J. Am. Soc. NephrolEpub ahead of print, 2016. doi:10.1681/ASN.2015070836.

Hussein, S., Zheng, W., Dyte, C., Wang, Q., Yang, J., Zhang, F., Tang, J., Cao, Y., and Chen, X.-Z.* Acid-induced off-response of PKD2L1 channel in Xenopus oocytes and roles of Ca2+. Sci. Rep5, 15752, 2015. DOI: 10.1038/srep15752.

Wentong Long, Pankaj Panwar, Kate Witkowska, Kenneth Wong, Debbie O’Neill, Xing-Zheng Chen, M. Joanne Lemieux, Chris I. Cheeseman. (2015) Critical Roles for Two Hydrophobic Residues within Human Glucose Transporter 9 (hSLC2A9) Affecting Substrate Selectivity and the Energetics of Urate Transport J Biol Chem. pii: jbc.M114.611178.

Wang, Q., Dai., X.-Q., Li, Q., Tuli, J., Liang, G., Li, S. and Chen, X.-Z.* (2013) Filamin interacts with ENaC and inhibits its channel function. J. Biol. Chem.288, 264-273.

Yang, J., Zheng, W., Wang, Q., Lara, C., Hussein, S., and Chen, X.-Z.* (2013)  Translational up-regulation of polycystic kidney disease protein PKD2 by endoplasmic reticulum stress. FASEB J. 27, 4998-5009.

Wang, Q., Dai, X.-Q., Wang, Z., Li, Q., Cantero, M.R., Li, S., Shen, J., Tu, J.-C., Cantiello, H.F. and Chen, X.-Z.* (2012) Structural interaction and functional regulation of polycystin-2 by filamin PLoS ONE. 7, e40448.

Yang, J., Wang, Q., Zheng, W., Tuli, J., Li, Q., Wu, Y., Hussein, S., Dai, X.-Q., Shafiei, S., Li, X.-G., Shen, P.Y., Tu, J.-C., and Chen, X.-Z.* (2012) Receptor for activated C kinase 1 (RACK1) inhibits the function of TRP-type channel Pkd2L1 through physical interaction. J. Biol. Chem. 287, 6551-6561.


Cordat, E.

Chang H.F., Bzeih H., Schirra C., Halamani M., Cordat E., Krause E., Pattu V., Rettig J. (2016) – “Endocytosis of cytotoxic granules is essential for multiple killing of target cells by T lymphocytes” Journal of Immunology, 197(6):2473-84. doi: 10.4049/jimmunol.1600828.

Alexander R.T., Cordat E., Chambrey R., Dimke H., Eladari D. (2016) – “Acidosis and urinary calcium excretion, insights from genetic disorders”. In press for Journal of the American Society of Nephrology.

Peleh, V., Cordat E., Herrmann JM. (2016) – “Mia40 is a trans-site receptor that drives protein import into the mitochondrial intermembrane space by hydrophobic substrate binding”, in press at eLife, doi: 10.7554/eLife.16177.

Almomani E, Lashhab R, Alexander RT, Cordat E. The carboxyl-terminally truncated kidney anion exchanger 1 R901X dRTA mutant is unstable at the plasma membrane. Am J Physiol Cell Physiol. 2016 May 1;310(9):C764-72. doi: 10.1152/ajpcell.00305.2015. Epub 2016 Mar 16.

Zheng W., Wang Q., Roy B, Zhang F, King J, Sergi C., Cordat E., Tang J., Cao Y., Ali D.W., and Chen X.Z. (2016) – “Identification of FUBP1 as a PKD2 mRNA 3’UTR binding protein that suppresses its translation”. Journal of the American Society of Nephrology. pii: ASN.2015070836.

Cordat E.* (2015)- “Setting up an academic research laboratory in Canada in 2015”. Canadian Journal of Kidney health and Disease. 2:51, doi: 10.1186/s40697-015-0086-1.

Krishnan D, Liu L, Wiebe SA, Casey JR, Cordat E, Alexander RT. (2015) Carbonic anhydrase II binds to and increases the activity of the epithelial sodium-proton exchanger, NHE3. Am J Physiol Renal Physiol. 309(4):F383-92.

Cordat, E.  and Reithmeier, R.A. (2014) Structure, function, and trafficking of SLC4 and SLC26 anion transporters.  Curr. Top Membr. 78:1-67.

Chu, C.Y., King, J.C., Berrini, M., Alexander, R.T., and Cordat, E. (2013) Functional rescue of a kidney anion exchanger 1 trafficking mutant in renal epithelial cells.  PLoS One8(2):e57062.

Almomani, E.Y., King, J., NetsawangJ., Yenchitsomanus, P.T., Malasit, P., Limjindapron, T., Alexander, R.T., and Cordat, E. (2012) Adaptor protein 1 complexes regulate intracellular trafficking of the kidney anion exchanger 1 in epithelial cells. American Journal of Physiology, Cell Physiology 303(5):C554-66.

Pan, W., Borovac, J., Spicer, Z., Hoenderop, J.G., Bindels, R.J., Schull, G.E., Doschak, M.R., Cordat, E. and RT. Alexander, R.T.  (2011) The epithelial sodium-hydrogen exchanger, NHE3, is necessary for renal and intestinal calcium (re)absorption. Am J Physiol Renal Physiol, 2(8):F943-56.

Almomani E., Chu C. and Cordat E. (2011) Mis-trafficking of bicarbonate transporters: implications to human diseases. Biochemistry and Cell Biology89(2):157-77.


Fliegel, L.

X. Li,A. Augustine, S.Chen, L. Fliegel. Stop Codon Polymorphisms in the Human SLC9A1 Gene Disrupt or Compromise Na+/H+ Exchanger Function. (2016) PLoS ONE 11(9): e0162902. doi:10.1371/ journal.pone.0162902

SR Amith, Krista Marie Vincent, Jodi Marie Wilkinson, Lynne Marie Postovit, and Larry Fliegel. (2017) Defining the Na+/H+ Exchanger NHE1 interactome in triple-negative breast cancer cells. Cellular Signalling 29:69-77. DOI 10.1016/j.cellsig.2016.10.005

Larry Fliegel. (2017) β-Raf Activation of the Myocardial Na+/H+Exchanger. Channels 11:3181-182. Invited Review.DOI: 10.1080/19336950.2016.1270150

SRAmith, L. Fliegel (2017) Na+/H+ exchanger-mediated hydrogen ion extrusion as a carcinogenic signal in triple-negative breast cancer etiopathogenesis and prospects for its inhibition in therapeutics. Seminars in Cancer Biology 43:35-41 DOI 10.1016/j.semcancer.2017.01.004,Nm413VrC

Ayo Odunewu-Aderibigbe and L. Fliegel  Protein Mediated Regulation of the NHE1 Isoform of the Na+/H+ Exchanger in Renal Cells. Cellular Signaling 2017 Aug;36:145-153. doi: 10.1016/j.cellsig.2017.05.003

Ayo Odunewu-Aderibigbe and L. Fliegel  Heat Shock Proteins and the Na+/H+ Exchanger. Channels, 2017 :1-3. doi: 10.1080/19336950.2017.1347001

S R Amith, Jodi Marie Wilkinson and L. Fliegel (2016) KR-33028, a potent inhibitor of the Na+/H+ Exchanger NHE1, suppresses metastatic potential of triple-negative breast cancer cells. Biochemical Pharmacology 118:31-39. DOI 10.1016/j.bcp.2016.08.010

Xiuju Li, A. Augustine, D. Sun, L. Li and L. Fliegel Activation of the Na+/H+ Exchanger in Isolated Cardiomyocytes Through b-Raf Dependent Pathways. Role of Thr653 of the Cytosolic Tail. (2016) J Mol Cell Cardiol 99:65-75.

X. Li, A. Augustine, S. Chen, L. Fliegel. Stop Codon Polymorphisms in the Human SLC9A1 Gene Disrupt or Compromise Na+/H+ Exchanger Function. (2016) PLoS ONE 11(9): e0162902. doi:10.1371/ journal.pone.0162902

SR Amith, Krista Marie Vincent, Jodi Marie Wilkinson, Lynne Marie Postovit, and Larry Fliegel. (2017) Defining the Na+/H+ Exchanger NHE1 interactome in triple-negative breast cancer cells. Cellular Signalling 29:69-77.

Amith, S. R., and Fliegel, L. (2016) The Na+/H+ exchanger in metastasis. Aging (Albany NY) 8, 1291. 27434610. 10.18632/aging.101002

Amith, S. R., Fong, S., Baksh, S., and Fliegel, L. (2015) Na (+)/H (+) exchange in the tumour microenvironment: does NHE1 drive breast cancer carcinogenesis? Int J Dev Biol 59, 367-377. 26679950. 10.1387/ijdb.140336lf

Amith, S. R., Wilkinson, J. M., and Fliegel, L. (2016) Na+/H+ exchanger NHE1 regulation modulates metastatic potential and epithelial-mesenchymal transition of triple-negative breast cancer cells. Oncotarget 7, 21091-21113. 27049728. 10.18632/oncotarget.8520

Ullah, A., Dutta, D., and Fliegel, L. (2016) Expression and characterization of the SOS1 Arabidopsis salt tolerance protein. Mol Cell Biochem 415, 133-143. 26992907. 10.1007/s11010-016-2685-2

Amith SR, Wilkinson JM and Fliegel L. Characterization of the role of phosphorylation sites in regulation of the Na+/H+ exchanger (NHE1) in triple-negative MDA-MB-231 breast cancer cells. In Press in Oncotarget.

Claire Guissart, Xiuju Li, Bruno Leheup, Nathalie Drouot, Philippe Jonveaux, Larry Fliegel, Michel Koenig. (2015) Mutation of SLC9A1, encoding the major Na+/H+ exchanger, causes ataxia-deafness Lichtenstein Knorr syndrome. Human Molecular Genetics 24(2):463-70. DOI 10.1093/hmg/ddu461.

Schammim Ray Amith, Jodi Marie Wilkinson, S. Baksh and Larry Fliegel (2015) The Na+/H+ exchanger (NHE1) as a novel co-adjuvant target in paclitaxel therapy of triple-negative breast cancer cells. Oncotarget 6(2):1262-75. Accession # 25514463 ISSN 1949-2553

Li, X. and Fliegel, L. (2015) A Novel Human Mutation in the SLC9A1 Gene Results in Abolition of Na+/H+ Exchanger Activity. Plos One 10(3) e0119453

Iman A. Mohamed, Alain-Pierre Gadeau, Gary Lopaschuk, Larry Fliegel, Mohamed Mlih, Nabeel Abdulrahman, Natasha Fillmore, Fatima Mraiche. Na+/H+ Exchanger Isoform 1-Induced Osteopontin Expression Facilitates Cardiomyocyte Hypertrophy Through p90 Ribosomal S6 Kinase. (2015) PLOS One 10(4) e0123318.

Amith, R.S, S. Fong, S. Baksh and L. Fliegel. Na+/H+ Exchange in the Tumour Microenvironment: Does NHE1 Drive Breast Cancer Carcinogenesis? International Journal of Developmental Biology. In Press March 23, 2015.

S.C. Guffey, L. Fliegel, G.G. Goss. (2015) Cloning and characterization of shark gill Na+/H+ Exchanger isoforms NHE2 and NHE3 from the Pacific dogfish Squalus suckleyi In Press in Comparative Biochemistry and Physiology – Part B: Biochemistry & Molecular Biology

  1. Liu, A. Basu, Xiuju Li and L. Fliegel. Topological Analysis of the Na+/H+ Exchanger. In Press BBA Biomembranes (July 22/2015)

Kemp, G., Fliegel, L., and Young, H.S.  (2014)  Membrane transport piece by piece:  production of transmembrane peptides for structural and functional studies.  Curr. Protoc. Protein Sci. 75:Unit 29 28.

Ullah, A., El-Magd, R.A., and Fliegel, L. (2014)  Functional role and analysis of cysteine residues of the salt tolerance protein Sod2.  Mol. Cell Biochem. 386:85-98.

Li, X., Prins, D., Michalak, M., and Fliegel, L. (2013)  Calmodulin-dependent binding to the NHE1 cytosolic tail mediates activation of the Na+/H+ exchanger by Ca2+ and endothelin.  Am. J. Physiol. Cell Physiol.  305:C1161-1169.

Ullah, A., Kemp, G., Lee, B.L., Alves, C., Young, H., Sykes, B.D. and Fliegel, L. (2013)  Structural and functional analysis of transmembrane segment IV of the salt tolerance protein Sod2.  J. Biol.Chem. 288:24609-24624.

Odunewu, A. and Fliegel, L. (2013)  Acidosis-mediated regulation of the NHE1 isoform of the Na(+)/H(+) exchanger in renal cells.  Am. J. Physiol. Renal Physiol. 305:F370-381.

Li, X., Khan, M.F., Schriemer, D.C., and Fliegel, L. (2013)  Structural changes in the C-terminal regulatory region of the Na(+)/H(+) exchanger mediate phosphorylation induced regulation.  J. Mol. Cell Cardiol. 61:153-163.

Amith, S.R. and Fliegel, L. (2013)  Regulation of the Na+/H+ Exchanger (NHE1) in Breast Cancer Metastasis.  Cancer Res.  73:1259-1264.

Lee, B.L., Sykes, B.D., and Fliegel, L. (2013)  Structural and functional insights into the cardiac Na(+)/H(+) exchanger.  J. Mol. Cell Cardiol. 61:60-67.

Blough, M.D., Al-Najjar, M., Chesnelong, C., Binding, C.E., Rogers,  A.D., Luchman, H.A., Kelly, J.J., Fliegel, L., Morozova, O., Yip, S., Marra, M., Weiss, S., Chan, J.A. and Cairncross, J.G.  (2012) DNA Hypermethylation and 1p Loss Silence NHE-1 in Oligodendroglioma.  Annals of Neurology 71(6):845-849.

Fliegel, L. Na+/H+ Exchangers, Structure, and Function: Role in Human Health and Disease. (2012) Encyclopedia of Metalloproteins. Springer Science.

Vanier, H.V., Mraiche, F., Li, X. and Fliegel, L. (2012) Gender specific effects of exercise on cardiac pathology in Na(+)/H(+) exchanger overexpressing mice. Mol Cell Biochem.  368:103-110.

Lee, B.L., Liu, Yonsheng, Li, X., Sykes, B.D. and Fliegel, L. (2012Structural and functional analysis of extracellular loop 4 of the NHE1 isoform of the Na+/H+ exchanger. Biochim. Biophys. Acta 1818:2783-2790.

Mraiche, F. and Fliegel, L.  (2011)  Elevated expression in activatred Na(+)/H(+) exchanger protein induces hypertrophy in isolated rate neonatal ventricular cardiomyocytes. Mol. Cell. Biochem. 358:179-187.

Tzeng, J., Lee, B.L., Sykes, B.D. and Fliegel, L. (2011)  Structural and functional analysis of critical amino acids in TMV1 of the NHE1 isoform of the Na+/H+ exchanger.  Biochim. Biophys. Acta 1808:2327-2335.

Lee, B.L., Sykes, B.D. and Fliegel, L.  (2011) Structural analysis of the Na+/H+ exchanger isoform 1 (NHE1) using  the divide and conquer approach.  Biochem. Cell Biol. 89:189-199.

Alexander, R.T. and Fliegel, L.  (2011)  Report on the Na+/H+ Exchanger Satellite Meeting at the 53rd Annual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology.  Biochem. Cell Biol 89:85-86.

Mraiche, F., Oka, T., Gan, X.T., Karmazyn, M. and Fliegel, L.  (2011) Activated NHE1 is required to induce early cardiac hypertrophy in mice. Basic Research Cardiology 106:603-616.

Karki, P., Li, X., Schrama, D. and Fliegel, L.  (2011) B-Raf associates with and activates the NHE1 isoform of the Na+/H+ exchanger.  J. Biol. Chem. 286(15):13096-13105.

Mraiche, F., Wagg, C.S., Lopaschuk, G.D. and Fliegel, L.  (2011) Elevated levels of activated NHE1 protect the myocardium and improve myocardial metabolism following ischemia/reperfusion injury. J. Mol. Cell. Cardiol. 50:157-164.


Kurata, H.T.

Kim, R.Y., Yau, M., Galpin, J.D., Ahern, C.A., Pless, S.A., and H.T. Kurata. 2015. Atomic basis for therapeutic activation of neuronal potassium channels. Nature Communications. 6:8116. PMID: 26333338.

Baronas, V., McGuinness, B.R., Brigidi, G.S., Gomm Kolisko, R.H., Vilin, Y.Y., Kim, R.Y., Lynn, F.C., Bamji, S.X., Yang, R.Y. and H.T. Kurata. 2015. Use-dependent activation of neuronal Kv1.2 channel complexes. J. Neurosci. 35(8):3515:3524. PMID: 25716850.

Pless, S.A., Kim, R.Y., Ahern, C.A., and H.T. Kurata. 2015. Atom-by-atom engineering of voltage-gated ion channels: magnified insights into function and pharmacology. J. Physiol. 593(12):2627-2634. PMID: 25640301.

Zhang, R.S., Wright, J., Pless, S.A., Nunez, J.J., Kim, R.Y., Li, J.B.W., Yang, R., Ahern, C.A., and  H.T. Kurata. 2015. A conserved residue that governs kinetics of ATP-dependent gating of Kir6.2 potassium channels. J. Biol. Chem. 290(25):15450-61. PMID: 25934393.

Pless, S.A., Galpin, J.D., Niciforovic, A.P., Kurata, H.T., and C.A. Ahern. 2013. Hydrogen bonds as molecular timers for slow inactivation in voltage-gated potassium channels. ELife, 2(0):e01289. PMID: 24327560.

Li, B.W., Huang, X., Zhang, R.S., Kim, R.Y., Yang, R., and H.T. Kurata. 2013. Decomposition of slide helix contributions to ATP-dependent inhibition of Kir6.2 channels. J. Biol. Chem.,288(32):23038-49. PMID: 23798684.

Pless, S.A., Niciforovic, A.P., Galpin, J.D., Nunez, J.J.Kurata, H.T., and C.A. Ahern. 2013. A novel mechanism for regulating open state stability in a voltage-gated potassium channel. Nature Communications, 4:1784).epub April 30, 2013.  PMID: 23653196


Lemieux, M.J.

Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1.
Xu Y, Chen G, Greer MS, Caldo KMP, Ramakrishnan G, Shah S, Wu L, Lemieux MJ, Ozga J, Weselake RJ.
J Biol Chem. 2017 Sep 12. pii: jbc.M117.811489. doi: 10.1074/jbc.M117.811489. [Epub ahead of print]
PMID: 289000

Diacylglycerol acyltransferase 1 is regulated by its N-terminal domain in response to allosteric effectors.

Caldo KMP, Acedo JZ, Panigrahi R, Vederas JC, Weselake RJ, Lemieux MJ.
Plant Physiol. 2017 Aug 21. pii: pp.00934.2017. doi: 10.1104/pp.17.00934. [Epub ahead of print]
PMID: 28827454

Wentong Long, Pankaj Panwar, Kate Witkowska, Kenneth Wong, Debbie O’Neill, Xing-Zheng Chen, M. Joanne Lemieux, Chris I. Cheeseman. (2015) Critical Roles for Two Hydrophobic Residues within Human Glucose Transporter 9 (hSLC2A9) Affecting Substrate Selectivity and the Energetics of Urate Transport J Biol Chem. pii: jbc.M114.611178.

Kristian Mark P. Caldo, Michael S. Greer, Guanqun Chen, M. Joanne Lemieux, Randall J. Weselake (2015) Solubilization and Purification of Recombinant Brassica napus Diacylglycerol Acyltransferase 1. FEBS Lett.;589(6):773-8.34.

Zoe Cournia, Tobby Allen, Ioan Andricioaei, Bruno Antonny, Daniel Baum, Grace Brannigan, Nicolae-Viorel Buchete, Jason T. Deckman, Lucie Delemotte, Coral del Val, Ran Friedman, Paraskevi Gkeka, Hans-Christian Hege, Jérôme Hénin, Marina Kasimova, Antonis Kolocouris, Michael L. Klein, Syma Khalid, M. Joanne Lemieux, Norbert Lindow, Mahua Roy, Jana Selent, Mounir Tarek, Florentina Tofoleanu, Stefano Vanni, Sinisa Urban, David J. Wales, Jeremy C. Smith, Ana-Nicoleta Bondar (2015) Membrane Proteins: Environmental Effects on Structure, Function and Dynamics. J Mol. 8(4):611-40.

Christopher Girke, Elena A Arutyunova, Maria Syed, Michaela Traub, Torsten Mohlmann, M. Joanne Lemieux. (2015) High yield purification and characterization of Equilibrative Nucleoside Transporter 7 (ENT7) from Arabidopsis thaliana. Biochim Biophys Acta 1850(9):1921-9.

Arutyunova, Panwar, P., Skiba, P.M., Gale, N., Mak,M.W., and Lemieux, M. Joanne  Allosteric regulation of rhomboid intramembrane proteolysis.  EMBOJ – in press.

Witz, S., Panwar, P., Schober, M., Deppe, J., Pasha, F.A., Lemieux, M. Joanne and Mohlmann, T.  (2014)  Structure-Function Relationship of a Plant NCS1 Member – Homology Modeling and Mutagenesis Identified Residues Critical for Substrate Specificity of PLUTO, a Nucleobase Transporter from Arabidopsis.  PLoS One, Mar 12;9(3):e91343.  doi: 10.1371/journal.pone.0091343.  eCollection 2014.

Brooks, C.L., Morrison, M., and Lemieux, M. Joanne (2013)  Novel plate screening technique for over-expression assessment of eukaryotic targets expressed in Pichia. Protein Science, 21 FEB 2013, DOI: 10.1002/pro.2223.

Lazareno-Saez, C., Arutyunova, E., Coquelle, N., and Lemieux, M. Joanne (2013)  Domain swapping in the cytoplasmic domain of E.coli rhomboid protease.  J. Mol. Biol. April 12;425(7):1127-1142.  doi: 10.1016/j.jmb.2013.01.019.

Padmapriya Sampathkumar, Michelle W. Mak, Sarah Withold, Emmanuel Guigard, Cyril M. Kay, M. Joanne Lemieux. (2012) Quaternary structure analysis of rhomboid intramembrane peptidases reveals a homodimer. BBA Biomembranes.(12):3090-3097.

Brooks, C.L., Lazareno-Saez, C., Lamoureux, J.S., Mak, M.W., and Lemieux, M.J. (2011)  Insights into substrate gating in H. influenzae rhomboid.  J. Mol. Biol. 407:687-697.


Leslie, E.M.

Possamai, L.A., McPhail, M.J., Quaglia, A., Zingarela, V., Abeles, R.D., Tidswell, R., Puthucheary, Z., Rawal, J., Karvellas, C.J., Leslie, E.M., Hughes, R.D., Ma, Y., Yassem, W., Shawcross, D.L. Bernal, W., Dharwan, A., Heaton, N.D., Thursz, M., Wendon, J.A., Mitry, R.R., and Antoniades, C.G.  (2013)  Character and Temporal Evolution of Apoptosis in Acetaminophen-induced Acute Liver Failure.  Crit. Care Med.  41:2543-2550.

Leslie, E.M.   (2012)  Arsenic Glutathione Conjugate Transport by the Human Multidrug Resistance Proteins (MRPs/ABCCs).  J. Inorg. Biochem. 108:141-149 (invited review).


Naranmandura, H., Carew, M.W., Lee, J., Leslie, E.M., Weinfeld, M., and Le, X.C.  (2011)  Comparative Toxicity of Arsenic Metabolites in Human Bladder Cancer EJ-1 Cells.  Chem. Res. Toxicol. 24:1586-2596.

Qazi, S.S., Osoria Pérez, A., Sam, M. and Leslie, E.M.  (2011)  Glutathione Transferase P1 (GSTP1) Interacts Strongly with the Inner Leaflet of the Plasma Membrane.  Drug. Metab. Dispos. 39:1122-1126. 


Carew, M.W., Naranmandura, H., Shukalek, C.B., Le, X.C., and Leslie, E.M.  (2011)  Monomethylarsenic Diglutathione [MMAIII(GS)2] Transport by the Human Multidrug Resistance Protein 1 (MRP1/ABCC1). Drug Metab. Dispos.  39:2298-2304.


Overduin, M.

Salim M, Knowles TJ, Hart R, Mohammed F, Woodward MJ, Willcox CR, Overduin M, Hayday AC, Willcox BE. (2016) Characterisation of a putative receptor binding surface on Skint-1, a critical determinant of dendritic epidermal T cell selection, J. Biol. Chemin press. PMID: 26917727.

Fogl C, F Mohammed, C Al-Jassar, M Jeeves, S White, M Overduin and M Chidgey, Molecular mechanism for intermediate filament recognition by plakin proteins, Nature Communications, 7:10827. PMID: 26935805

Browning DF, Bavro VN, Mason J, Sevastsyanovich YR, Rossiter AE, Jeeves M, Wells TJ, Knowles T, Cunningham AF, Donald J, Palmer T, Overduin M, Henderson IR (2015) Cross-species chimeras reveal BamA POTRA and β-barrel domains must be fine-tuned for efficient OMP insertion, Mol Micro, 97(4):646-59

Lenoir M, M Grzybek, M Majkowski, S Rajesh, J Kaur, Ü Coskun and M Overduin (2015) Structural basis of dynamic membrane recognition by trans-Golgi network specific FAPP proteins, J Mol Biol. 427(4):966-81.

Jeeves M, L Quill, M Overduin, Structure-based Drug Design using NMR (2015) eMagRes 4, 231–240.

Kufareva I, M Lenoir, F Dancea, P Sridhar, E Rausch, C Bissig, J Gruenberg, R Abagyan, M Overduin (2014) Discovery of novel membrane binding structures and functions, Biochem Cell Biol, 92(6):555-563.

RK Koppisetti, YG Fulcher, A Jurkevic, SH Prior, J Xu, M Lenoir, M Overduin, and SR Van Doren (2014) Ambidextrous Binding of Membrane Bilayers by a Soluble Metalloproteinase, Nature Comms. 5:5552.

Lenoir, M, M Sugawara, J Kaur, LJ Ball, M Overduin (2014) Structural Insights into the Activation of the RhoA GTPase by the Lbc Oncoprotein, J Biol Chem, 289(34):23992-4004.

Butterworth S, Overduin M and Barr AJ (2014) Targeting Protein Tyrosine Phosphatase SHP2 for therapeutic intervention in cancer Future Med Chem 6(12):1423-37. PMID: 25329198.

Al-Jassar C, Bikker H, Overduin M, Chidgey M (2013) Mechanistic basis of desmosome-targeted diseases, J Mol Biol, 425(21):4006-22.

Al-Jassar C, Bernado, P, Chidgey M, Overduin M (2013) Hinged plakin domains provide specialized degrees of articulation in envoplakin, periplakin and desmoplakin, PLoS ONE 8(7): e69767. PMID: 23922795

Bissig C, M Lenoir, MC Velluz, I Kufarena,  R Abagyan, M Overduin, J Gruenberg* (2013) Viral infection controlled by calcium dependent recognition of late endosome-specific lipid by the Alix Bro1 domain, Dev Cell 25:364-73.

Lenoir, M and M Overduin (2013) PtdIns(4)P signalling and recognition sytems, Adv Exp Med Biol 991:59-83.

Rajesh S, P Sridhar, BA Tews, L Fénéant, L Cocquerel, D Ward, F Berditchevski, M Overduin (2012) Structural basis of ligand interactions of the large extracellular domain of tetraspanin CD81, J Virol 86(18):9606-16.

Rajesh S, Knowles TJ, Overduin M (2011)Production of membrane proteins without cells or detergents, New Biotechnology, 28(3):250-4.

Knowles, TJ, DF Browning, M Jeeves, R Maderbocus, S Rajesh, P Sridhar, E Manoli, D Emery, U Sommer, A Spencer, DL Leyton, D Squire, RR Chaudhuri, MR Viant, AF Cunningham, IR Henderson, M Overduin (2011) Structure and function of BamE within the outer membrane and the β-barrel assembly machine, EMBO Reports 12(2):123-8.

Rajesh S, Bago R, Odintsova E, Muratov G, Sridhar P, Rajesh S, Overduin M, Berditchevski F (2011) Binding to syntenin-1 defines a new mode of ubiquitin-based interactions regulated by phosphorylation, J Biol Chem. 286(45):39606-14.

Al-Jassar C, Knowles T, Jeeves M, Kami K, Bikker H, Overduin M, Chidgey M (2011) The nonlinear structure of the desmoplakin plakin domain and the effects of cardiomyopathy-linked mutations, J Mol Biol, 411(5):1049-61.

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Touret, N.

Lipinski, T.*, Fitieh, A.*, St. Pierre, J., Ostergaard, H.L., Bundle, D.R. and Touret, N. (2013) Enhanced Immunogenicity of a tricomponent mannan tetanus toxoid conjugate vaccine targeted to DCs via Dectin-1 by incorporating b-glucan.  J Immunol. [Epub ahead of print]

Goodridge, H. S., Underhill, D. M., & Touret, N. (2012). Mechanisms of fc receptor and dectin-1 activation for phagocytosis. Traffic13(8), 1062–1071.

Haile Y., Simmen K.C., Pasichnyk D., Touret N., Simmen T., Lu J.Q., Bleackley R.C., Giuliani F .  (2011)  Granule-Derived Granzyme B Mediates the Vulnerability of Human Neurons to T Cell-Induced Neurotoxicity.  J Immunol9(187):(4861-72). Impact Factor: 5.75

Prins D., Groenendyk J., Touret N, Michalak M.   (2011)  Modulation of STIM1 and capacitative Ca(2+) entry by the endoplasmic reticulum luminal oxidoreductase ERp57. EMBO Rep. 11(12):(1182-8). Impact Factor: 7.82

Jaqaman K., Kuwata H., Touret N, Collins R., Trimble W.S., Danuser G., Grinstein S.  (2011)  Cytoskeletal control of CD36 diffusion promotes its receptor and signaling function. Cell146(4):(593-606). Impact Factor: 32.4

Weiner, JH

Prehna, G., Zhang, G., Gong, X., Dusyk, M., Okon, M. McIntosh, L.P., Weiner, J.H. and Strynadka, N.C.J. (2012)  Protein Export via the Escherichia coli Porin Channel. Structure 20:1154-1166.

Tran, Q.M., Fong, C., Rothery, R.A., Maklashina, E., Cecchini, G. and Weiner, J.H. (2012)  Out of plane distortions of the heme b of Escherichia coli succinate dehydrogenase PLOS One 7:e32641.

Rothery, R.A., Stein, B., Solomonson, M.W., Kirk, M.L. and Weiner, J.H. (2012)  Pyranopterin conformation defines the function of molybdenum and tungsten enzymes Proc. Natl. Acad. Sci (USA) 109:14773-14778.

Cherney, M.M., Zhang, Y., James, M.N.G., and Weiner, J.H. (2012)  Structure-activity characterization of sulfide:quinone oxidoreductase variants J. Struct. Biol. 178:319-328.

Tang, H., Rothery, R.A., Voss, J. E. and Weiner, J.H.  (2011)  Correct Assembly of FS0 into the Escherichia coli DMSO Reductase (DmsABC) is a Pre-requisite for Molybdenum Cofactor Insertion. J. Biol. Chemß:15147-15154.


Young, HS

Gorski, P.A.Trieber, C.A., Ashrafi, G.Young, H.S. (2015) Regulation of the sarcoplasmic reticulum calcium pump by divergent phospholamban isoforms in zebrafish. Journal of Biological Chemistry 290:6777-88.

Young, H.S.Ceholski, D.K.Trieber, C.A. (2015) Deception in simplicity: Hereditary phospholamban mutations in dilated cardiomyopathy. Biochemistry and Cell Biology 93:1-7.

Abrol, N., Smolin, N., Armanious, G.Ceholski, D.K.Trieber, C.A.Young, H.S., Robia, S.L. Phospholamban C-terminal residues are critical determinants of the structure and function of the calcium ATPase regulatory complex. Journal of Biological Chemistry 289:25855-66.

Muller, M., Kunz, H.H., Schroeder, J., Kemp, G.Young, H.S., and Neuhaus, E.  (2014)  Decreased capacity of sodium import into Arabidopsis chloroplasts impairs salt tolerance, photosynthesis and plant performance.  The Plant Journal (in press).

Kemp, G., Fliegel, L., and Young, H.S. (2014)  Membrane Transport Piece by Piece: Production of Transmembrane Peptides for Structural and Functional Studies.  Current Protocol in Protein Science Curr Protoc Protein Sci. 2014 Feb 3;75:Unit 29.8.

Ullah, A., Kemp, G., Lee, G., Alves, C., Young, H.S., Sykes, B.D., and Fliegel, L.  (2013)  Structural and functional analysis of transmembrane segment IV of the salt tolerance protein sod2.  Journal of Biological Chemistry  288:24609-24624.

Gorski, P.A.Glaves, J.P., Vangheluwe, P., and Young, H.S. (2013)  Sarco/endoplasmic reticulum calcium ATPase (SERCA) inhibition is encoded in its luminal tail.  Journal of Biological Chemistry 288:8456-8467.

Smeazzetto, S., Saponaro, A., Young, H.S., Monicelli, M.R., and Thiel, G.  (2013)  Structure-function relation in phospholamban:  Modulation of channel activity as a potential regulator of SERCA activity.  PLoS One 8:(1):e52744.

Cortez, M., Kumar, J., Renault, L.Young, H.S., and Sim,  V.L.  (2013)  Mouse prion protein polymorphism 108F/189V affects the kinetics of fibril formation and the response to seeding; evidence for a two-step nucleation polymerization mechanism.  Journal of Biological Chemistry 288:4772-4781.

Glaves, J.P.Gorski, P.A., Alier, K., Ma, L., Primeau, J.O., Jhamandas, J.H., and Young, H.S.  (2013)  Distinct morphological and electrophysiological properties of an elk prion peptide.  Peptides  40:49-56.

Ceholski, D.K.Trieber, C.A., and Young, H.S.  (2012)  Hydrophobic imbalance in the cytoplasmic domain of phospholamban is a determinant for lethal dilated cardiomyopathy.  Journal of Biological Chemistry  287:16521-16529.

Ceholski, D.K.Trieber, C.A., Holmes, C.F., and Young, H.S.  (2012)  Lethal, hereditary mutants of phospholamban elude phosphorylation by protein kinase A.  Journal of Biological Chemistry  287:26596-26605.

Gorski, P.A., Trieber, C.A., Lariviere, E., Schuermans, M., Wuytack, F., Young, H.S. and Vangheluwe, P.  (2012)  Transmembrane helix 11 is a genuine regulator of the endoplasmic reticulum Ca2+ pump and acts as a functional parallel of b-subunit on a-Na+, K+-ATPase.  Journal of Biological Chemistry  287:19876-19885.

Chaulk, S., Thede, G.L., Kent, O.A., Xu, Z., Gesner, E., Veldhoen, R.A., Khanna, S.K., Goping, I.S., MacMillan, A.M., Mendell, J.T., Young, H.S., Fahlman, R.P., Glover, J.N.M. (2011)  Role of pri-miRNA structure in miR-17~92 biogenesis. RNA Biology  8:1105-14.


Misono, K., Philo, J., Arakawa, T., Ogata, C., Qiu, Y., Ogawa, H., Young, H.S. (2011) Structure, signaling mechanism and regulation of natriuretic peptide receptor-guanylate cyclase. FEBS Journal 278:1818-29.3.

Glaves, J.P.Trieber, C.A.Ceholski, D.K., Stokes, D.L., Young, H.S. (2011) Phosphorylation and mutation of phospholamban alter physical interactions with the sarcoplasmic reticulum calcium pump. Journal of Molecular Biology 405:707-23.


Young, JD

Damaraju, V, Surapaneni, S, Kumar, G, Ng, AM, Yao, SYMYoung, JD, Cass, CE and Tong, Z. (2012). Role of human nucleoside transporters in the uptake and cytotoxicity of azacitidine and decitabine.  Nucleosides, Nucleotides & Nucleic Acids 31:236-255.

Huysmans, GH, Chan, N, Baldwin JM, Postis, VL, Tzokov, SB, Deacon, SE, Yao, SYMYoung, JD, McPherson, MJ, Bullough, PA, Baldwin, SA. (2012). A urea channel from Bacillus cereus reveals a novel hexameric structure.  Biochem. J. 445:157-166.

Witkowska K, Smith, KMYao, SYM, Ng, AML, O.Neill, D, Karpinski, E, Young, JD, Cheeseman, CI.  (2012). Human SLC2A9a and SLC2A9b isoforms mediate electrogenic transport of urate with different characteristics in the presence of hexoses. Am. J. Physiol. Renal Physiol. 303:F527-539.

Vaziri, H, Baldwin, SA, Baldwin JM, Adams DG, Young, JD, Postis, VL. (2012). Use of molecular modelling to probe the mechanism of the nucleoside transporter NupG. Mol. Membr. Biol. [Epub ahead of print, Dec. 21] .

Damaraju, V.L., Smith, K.M., Mowles, D., Nowak, I., Karpinski, E., Young, J.D., Robins, M. and Cass C.E.  Interaction of fused-pyrimidine nucleoside analogs with human concentrative nucleoside transporter 1.  Mol. Pharmacol. 81:82-90 (2011). PubMed Accession Number: 20854794.  [Journal Impact factor 4.53]

Graham, K., Yao, S.Y.M., Johnson, L., Mowles, D., Ng, A.M.L., Wilkinson, J., Young, J.D. and Cass, C.E.  Nucleoside transporter gene expression in wild-type and mENT1 knockout mice.  Biochem. Cell Biol. 89:236-2145 (2011).  PubMed Accession Number: 21455274. [Journal Impact factor 2.61]

Kost, S., Sun, C., Graham, K., Cass, C.E., Young, J.D., Albensi, B.C. and Parkinson, F.E.  Behavioral effects of elevated expression of human equilibrative nucleoside transporter 1 in mice.  Behav. Brain Res. 224:44-49 (2011).  PubMed Accession Number: 21645551.  [Journal Impact factor 3.22]

Yao, S.Y.M., Ng, A.M.L., Cass, C.E., Baldwin, S.A. and Young, J.D.  Nucleobase transport by human equilibrative nucleoside transporter 1 (hENT1).  J. Biol. Chem.  286:32552-32562 (2011).  PubMed Accession Number: 21795683.  [Journal Impact factor 5.52]

Damaraju, V.J., Mowles, D., Smith, K.M., Yao, S.Y.M., Young, J.D., Marquez, V.E. and Cass, C.E.  Influence of sugar ring conformation on transportability of nucleosides by human nucleoside transporters.  Chem. Bio. Chem. [Epub ahead of print, Nov. 4, 2011].  PubMed Accession Number: 22052809.  [Journal Impact factor 3.82]